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Aim
To identify the chemical characters of the given sample.
Chemical Tests and Observations
| Test | Observation | Inference |
|---|---|---|
| Fiehe’s test: Take about 3ml of honey + 2ml of ether and shake thoroughly and allow the 2 layers to separate and evaporate to dryness. The upper etherial layer is separated and put in a china dish and evaporate, to the residue add 1% resorcinol and HCl. | Transient pink colour Permanent red colour | Pure honey Adulterated honey(Invert sugar) |
| Molisch’s Test: Honey is treated with alpha Naphthol and concentrated sulphuric acid | Purple colour | Presence of carbohydrate |
| Reducing Sugar Test: Heat honey to this add a drop of a mixture of Fehling’s solution A & B | The brick red colour of cuprous oxide | Presence of monosaccharide |
Conclusion
From the above morphological characters and chemical tests, the given crude drug is identified as Honey.
References
1.Kokate CK. Practical Pharmacognosy, 4 edition, VallabhPrakashan. Delhi; 1994: 98
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